| Data | In casa | Ospite | Risultato |
|---|---|---|---|
| 27/10/26 18.30 | CD Cuarte | FC Girona | 0:2 |
| 27/10/26 19.00 | Sestao River Club | Espanyol Barcellona | 4:3d.t.s |
| 27/10/26 19.00 | CF Calamocha | CE Europa | 0:3 |
| 27/10/26 19.00 | Atletico Pinatarense | FC Elche | 1:3 |
| 27/10/26 19.45 | UD Castellonense | UD Almeria | 1:2d.t.s |
| 27/10/26 20.00 | Atletico Tordesillas | CD Mirandes | 5:7d.c.r |
| 27/10/26 20.00 | CF Lucena | CF Rayo Majadahonda | 0:1 |
| 27/10/26 20.00 | Gimnastica Segoviana | CyD Leonesa | 0:1 |
| 27/10/26 20.00 | UD Llanera | SD Ponferradina | 4:6d.c.r |
| 27/10/26 20.00 | RSD Alcalá | UD Las Palmas | 1:3d.t.s |
| 27/10/26 20.30 | CD Don Benito | CD Extremadura | 2:4d.t.s |
| 27/10/26 20.30 | CE Manresa | UE Sant Andreu | 3:6d.c.r |
| 27/10/26 21.00 | Arosa SC | CF Pontevedra | 2:0 |
| 27/10/26 21.00 | CD Atletico Paso | Cordoba CF | 3:1 |
| 27/10/26 21.00 | CD Tudelano | Real Saragossa | 0:4 |
| 27/10/26 21.00 | CD Manacor | FC Villarreal | 0:5 |
| 27/10/26 21.00 | Recreativo Huelva | Rayo Vallecano | 2:3d.t.s |
| 27/10/26 21.00 | Sporting de Alcazar | FC Malaga | 0:3 |
| 29/10/26 18.00 | CD Numancia | Real Oviedo | 7:5d.c.r |
| 29/10/26 18.00 | FC Pena Sport | SD Huesca | 1:5 |
| 29/10/26 19.00 | Atletico Baleares | CD Eldense | 0:1 |
| 29/10/26 19.00 | San Sebastian de los Reyes | CD Coria | 1:2d.t.s |
| 29/10/26 19.00 | FC Coruxo | Zamora CF | 0:2 |
| 29/10/26 19.00 | UD Poblense | AD Ceuta FC | 1:2 |
| 29/10/26 19.00 | Atletico Calatayud | CA Osasuna | 0:6 |
| 29/10/26 19.00 | UB Lebrijana | FC Getafe | 2:3 |
| 29/10/26 19.30 | CD Cieza | FC Granada | 0:3 |
| 29/10/26 20.00 | Antequera CF | CDA Aguilas FC | 2:0 |
| 29/10/26 20.00 | CD Varea | Andorra CF | 0:3 |
| 29/10/26 20.00 | Ub Conquense | CD Castellon | 0:3 |
| 29/10/26 20.00 | CD Covadonga | Real Valladolid | 0:1 |
| 29/10/26 20.00 | CD Laredo | Burgos CF | 2:4 |
| 29/10/26 20.00 | CD Guijuelo | Sporting Gijon | 1:3 |
| 29/10/26 20.00 | Real Ávila CF | Unionistas de Salamanca | 1:0d.t.s |
| Annullata | Mijas-Las Lagunas | Real Jaén C.F. | -:- |
| 29/10/26 20.30 | RS Gimnastica | UD Ourense | 3:1d.t.s |
| 29/10/26 21.00 | Deportivo Xerez | FC Cadiz | 1:3 |
| 29/10/26 21.00 | Auriense CA | Deportivo La Coruna | 0:3 |
| 29/10/26 21.00 | Ribadesella CF | Racing Santander | 0:2 |
| 29/10/26 21.00 | Sporting Hortaleza | FC Siviglia | 0:3 |
| 29/10/26 21.00 | AE Prat | Athletic Bilbao | 1:6 |
| 03/11/26 19.00 | CD Anaitasuna | Deportivo Alaves | -:- |
| 03/11/26 19.00 | CD Pamplona | SD Eibar | -:- |
| 04/11/26 19.00 | Club Portugalete | Real Union Club | -:- |
| 04/11/26 20.00 | SD Amorebieta | UD Logroñés | -:- |
| 04/11/26 20.30 | Utebo | CD Sabadell | -:- |
| 04/11/26 20.30 | Deportiva Minera | FC Cartagena | -:- |
| 04/11/26 20.30 | CF Reus Deportiu | Barakaldo CF | -:- |
| 04/11/26 20.45 | CS Puertollano | Albacete Balompie | -:- |
| 04/11/26 21.00 | CD Badajoz | CD Teneriffa | -:- |
| 05/11/26 19.00 | CD Tarancon | UD Levante | -:- |
| 05/11/26 21.00 | CD Alcoyano | RCD Mallorca | -:- |
| 10/11/26 20.00 | Trival Valderas | CD Leganes | -:- |
| 10/11/26 21.00 | Tavernes de la Valldigna | FC Valencia | -:- |
| 12/11/26 19.00 | CD Artistico Navalcarnero | Real Betis Siviglia | -:- |
| 13/11/26 21.00 | Salamanca CF UDS | Celta Vigo | -:- |